{"id":804,"date":"2023-01-28T18:20:58","date_gmt":"2023-01-28T18:20:58","guid":{"rendered":"https:\/\/dev1992.com\/?p=804"},"modified":"2023-01-28T18:45:01","modified_gmt":"2023-01-28T18:45:01","slug":"practical-bacteriology-from-scratch","status":"publish","type":"post","link":"https:\/\/dev1992.com\/index.php\/2023\/01\/28\/practical-bacteriology-from-scratch\/","title":{"rendered":"Cup\u00f3n Udemy: practical bacteriology from scratch"},"content":{"rendered":"\n
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you should know what will you do in lab<\/h2>\n \n\n \n
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practical bacteriology from scratch<\/figcaption><\/figure><\/div>\n \n\n \n
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practical bacteriology from scratch\u00a0 your safety first\u00a0 \u00a0take care when working with pathogenic microorganisms wear coat\u00a0 when you are in lab and remove after finish keep the lab disk for equipment only wear gloves when dealing with microorganisms clean\u00a0 clean\u00a0 table before and after test mask eye protect\u00a0 keep your hand away from mouth and eye and nose\u00a0 use mechanical pipetting report the spill and disinfect the area well\u00a0 wash hands after any procedure deal well with fire smooth small one with towel large one use expenditure follow the instructions included in lab safety manual be professional in handling microscope\u00a0 there are many types with many uses bright field microscope\u00a0 dark field microscope phase contrast microscope fluorescent microscope electro microscope\u00a0 it is essential tool practice using the microscope with low power medium power\u00a0 high power and take care when use oil immersion lens sterilization the most important topic in microbiology\u00a0 without keeping your environment sterile it is impossible to obtain Apure culture\u00a0 sterilization methods heat sterilization\u00a0 sterilization by radiation sterilization by disinfectant chemicals\u00a0 \u00a0use of antimicrobial bacterial culture is a\u00a0 method for isolate bacteria the culture may be natural in origin or synthetic or both culture may be ordinary enriched\u00a0 \u00a0 \u00a0 blood agar is enriched media They contain substances Blood ,Serum. Extract of plants or animal tissues that enhance growth of bacteria\u00a0 \u00a0Heated blood agar chocolate agar \u25aa heated blood agar chocolate agar heating blood \u25aa in water path at 75c for 10 minutes \u25aa Until blood become chocolate brown in color\u00a0 \u00a0 \u00a0 differential or specific\u00a0 and see it in colonies which in appearance characteristic after culture we need to sea the bacterial cell clearly stain\u00a0 our tool to change the background and sea bacterial morphology and enhance the contrast we sea details\u00a0 differential or structural preparing the smear and fix on Bunsen flame\u00a0 \u00a0simple stain like methylene blue crystal violet negative staining is simplest method\u00a0 \u00a0differential stain differentiate the bacteria into two groups\u00a0 like gram stain differentiate bacteria into gram +ve gram -ve\u00a0 it is invented by hans gram he use methylene blue as basic stain iodine as\u00a0 \u00a0bacteria are not decolorized and remain violet after decolorization carbol fuchsine a red counter stain is used to impart a pink color to the decolorized gram negative organisms The acid fast stain is a different stain in 1882 Paul Ehrlich discovered mycobacterium tuberculosis the causative agent of tuberculosis retained the primary stain even after washing with an acid alcohol mixture most bacteria are decolorized by acid alcohol only family mycobacteriacae and nocardiacea The cell wall of acid fast contain wax like lipid called mycolic acid render the cell impermeable to most stain technique developed by franz zeil and fredrch neelsen capsule stain Some bacteria are surrounded by slimy layer called capsule some glycoprotein some polypeptide all appear water soluble heat fixed smear destroy the capsule if not fixed slide from slide combine negative and simple staining species of bacteria related to genera bacillus or clostridium produce heat resistant structure called spores resistance to chemicals and heat due to thick tough spore coat\u00a0 Schaeffer- Fulton methods utilize malachite green to stain the endospores and safranin or carbol fuchsin to stain the vegetative portion of the cell green spore contained in pink vegetative bacilli\u00a0 bacteria to continue its life and defend herself produce enzymes like oxidases catalase coagulase we use these enzyme in identifying every bacteria biochemical test are designed for this purpose step by step you can be bacteriologist\u00a0 just keep goingWho this course is for:medical students study either medicine pharmacy dentistry veterinary medicine science secondary school prepare themselves to study medicine or become science students prepare themselves to study medicine or scienceany one interested of microbiology<\/p>\n \n\n \n

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